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:Super High Fidelity DNA Polymerase

 Super High Fidelity DNA Polymerase

Description
The Super Fidelity DNA Polymerase is a kinds of fast, high amplification efficiency and high fidelity DNA Polymerase. This Polymerase has the 5′to 3′DNA polymerase activity and 3′to 5′exonuclease activity. The modified Super Fi Polymerase, it’s activity area fused polymeric enhanced area, the special structure promote the fidelity and amplification efficiency greatly. The fidelity of Super Fi polymerase is the 50 times of the general Taq Polymerase and the 6 times of the Pfu polymerase. The amplification speed is 15-30 sec/KB, which successfully overcome the low amplification efficiency and low amplification speed defect of Pfu, reduce much of the reaction time.

This enzyme especially applicable for the long DNA fragment clone, especially have a great advantage in >10KB fragment amplification, the amplification fragment can be as long as 20 KB.

The Super Fidelity Polymerase amplification products do not has the ”A” at the 3’ end, can be cloned in the blunt end vector. If want T/A clone, should add the “A” at the end of the PCR products. This Polymerase has the high amplification speed, high amplification efficiency and high fidelity characteristics. Applicable for the gene clone, site-specific mutagenesis  and SNP and so on.
 

Features:
High Fidelity: The fidelity of Super Fi polymerase is the 50 times of the Taq Polymerase and the 6 times of the Pfu polymerase.

High Speed:The amplification speed is 15-30 sec/KB, which successfully overcome the low amplification efficiency and low amplification speed defect of Pfu.

Especially for long fragment: The fragment can be as long as 20KB.

Concentration: 2 u/µl                        Package:Bulk

Storage:Store at-20°C

Specifications

General Specifications

 

Hot Start:

No

Fidelity (vs. Taq):

50 X

Polymerase:

Super Fidelity DNA Polymerase

Final Product Size(s):

20 kb or more

Reaction Format:

Separate Components

Reaction Speed:

15-30 sec/kb

GC-Rich PCR Performance:

Low

Product Size:

100 reactions

Product Overhang:

Blunt

Shipping Condition:

Dry Ice/Ice bag

Exonuclease Activity:

3' - 5'

Regulatory Statement:

For Research Use Only. Not for use in diagnostic procedures.

Reaction Mixture Set Up (20μl reaction)

 

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