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LA Taq:LA Taq DNA Polymerase

LA Taq DNA Polymerase

The LA Taq DNA Polymerase is a kinds of  Long PCR Polymerase which synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone. The fidelity of PCR with this enzyme is three times higher than with Taq DNA Polymerase. The LA Taq is optimized for generation of very long amplicons: up to 40 kb with viral DNA and up to 15 kb with genomic DNA templates. The specially formulated LA Taq Buffer protects DNA from depurination and nicking during long thermal cycling. The PCR products generated with the LA Taq DNA Polymerase are mostly 3'-dA tailed, which can be cloned in TA vector.

Features: • Long PCR products:

–– up to 40 kb with viral DNA as template

–– up to15 kb with genomic DNA as template

. • Ideal for GC-rich templates up to 85% GC.

• Fidelity is three-times higher than with Taq DNA Polymerase.

• High yields. • Incorporates modified nucleotides.

Concentration: 5 u/μl

Quality Control: Functionally tested in generation of 40 kb amplicon with lambda DNA as template.

Storage Buffer: The enzyme is supplied in: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; Stabilizers; 50% glycerol.

Storage: Store at -20°C.

Reaction PCR Mixture Set Up and Recommended thermal cycling conditions: The flowing is only an example which take a 20KB human genomic DNA as the template, that only for a reference, It can be adjusted according the length and the sequence of the template and the primer.

Component        Volume        Final Concentration 

Template DNA    <1 ug          as required

Forward Primer (10 μM) 1 μl 0.2-0.4 μM each

Reverse Primer (10 μM) 1 μl 0.2-0.4 μM each

10×LA Taq Buffer 5 μl 1×

2.5 mM dNTPs 4 μl 0.2 mM

LA Taq DNA polymerase 0.5μl 2.5 unit

ddH2O to final volume 50μl Not applicable

94℃ 3 min

94℃ 30 sec

55℃ 30 sec 30 cycles

72℃ 1 min

72℃ 5 min

 

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